Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation
نویسندگان
چکیده
منابع مشابه
Corrigendum to “Extraction of HCV-RNA from Plasma Samples: Development towards Semiautomation”
A semiautomated extraction protocol of HCV-RNA using Favorgen RNA extraction kit has been developed. The kit provided protocol was modified by replacing manual spin steps with vacuum filtration. The assay performance was evaluated by real-time qPCR based on Taqman technology. Assay linearity was confirmed with the serial dilutions of RTA (Turkey) containing 1 × (10(6), 10(5), 10(4), and 10(3)) ...
متن کاملTechnical Note: RNA Extraction from FFPE Samples
Existing methods rely primarily on heat to remove crosslinks and adducts, which is only partially effective and leads to additional fragmentation of labile nucleic acids such as RNA. In contrast, the catalytic CAT5TM technology developed by Cell Data Sciences and included in the RNAstormTM kit greatly accelerates the removal of formaldehyde damage and allows the use of milder conditions, result...
متن کاملImpact of RNA extraction from limited samples on microarray results.
To move microarray technology into the diagnostic realm, the impact of technical parameters, such as sample preparation and RNA extraction, needs to be understood and minimized. We evaluated the impact of two RNA extraction methods, DNase treatment and the amount of hybridized cDNA probe, on the outcome of microarray results. The results for both RNA extraction methods were comparable, although...
متن کاملOptimization of RNA Extraction from Rat Pancreatic Tissue
Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...
متن کاملOptimization of an RNA isolation procedure from plasma samples.
In this study, we aimed to optimize the isolation procedure of circulating RNA from large volumes of plasma. Simultaneously, the stability and integrity of RNA from plasma samples were examined. To investigate the isolation of circulating RNA, reverse transcription-PCR analysis in combination with capillary electrophoresis was used. The presence of amplifiable RNA in plasma from healthy volunte...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Analytical Chemistry
سال: 2015
ISSN: 1687-8760,1687-8779
DOI: 10.1155/2015/367801